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upright epifluorescent microscope  (Nikon)


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    Structured Review

    Nikon upright epifluorescent microscope
    Upright Epifluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/upright+epifluorescence+microscope/Objectives/bio_rxiv__64898__2026__02__12__705597-283-9-12
    Average 99 stars, based on 59676 article reviews
    upright epifluorescent microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension
    Article Snippet: All arteries used for experimentation had a luminal diameter ∼150 μm, and were perfused with PSS (37°C) at a rate of 1.5 ml min - using a Gilson Minipuls 3 peristaltic pump. .. Intact mesenteric artery endothelial cell Ca 2+ activity was recorded at 10 Hz using one of three imaging systems optimized for high-resolution Ca 2+ imaging: an upright epifluorescence microscope (described above; FN-1, Nikon, Japan) equipped with a 16X 0.8 NA objective lens, and two inverted fluorescence microscopes (TE300 or Ti-Eclipse, Nikon, Japan) each equipped with 40X and 100X oil-immersion (1.3 NA; S Fluor) objective. ..

    Imaging:

    Article Title: Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension
    Article Snippet: All arteries used for experimentation had a luminal diameter ∼150 μm, and were perfused with PSS (37°C) at a rate of 1.5 ml min - using a Gilson Minipuls 3 peristaltic pump. .. Intact mesenteric artery endothelial cell Ca 2+ activity was recorded at 10 Hz using one of three imaging systems optimized for high-resolution Ca 2+ imaging: an upright epifluorescence microscope (described above; FN-1, Nikon, Japan) equipped with a 16X 0.8 NA objective lens, and two inverted fluorescence microscopes (TE300 or Ti-Eclipse, Nikon, Japan) each equipped with 40X and 100X oil-immersion (1.3 NA; S Fluor) objective. ..

    Microscopy:

    Article Title: Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension
    Article Snippet: All arteries used for experimentation had a luminal diameter ∼150 μm, and were perfused with PSS (37°C) at a rate of 1.5 ml min - using a Gilson Minipuls 3 peristaltic pump. .. Intact mesenteric artery endothelial cell Ca 2+ activity was recorded at 10 Hz using one of three imaging systems optimized for high-resolution Ca 2+ imaging: an upright epifluorescence microscope (described above; FN-1, Nikon, Japan) equipped with a 16X 0.8 NA objective lens, and two inverted fluorescence microscopes (TE300 or Ti-Eclipse, Nikon, Japan) each equipped with 40X and 100X oil-immersion (1.3 NA; S Fluor) objective. ..

    Article Title: Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
    Article Snippet: .. The retinas were double-labeled with anti- RNA-binding protein with multiple splicing (RBPMS, 1:500 dilution, GeneTex, Irvine, CA, USA) and anti-beta-tubulin III (TUJ1, 1:1000 dilution, BioLegend, San Diego, CA, USA) primary antibodies, incubated in appropriate secondary antibodies (1:1000 dilution donkey anti-rabbit 555, and 1:1000 dilution donkey anti-mouse 488, ThermoFisher, Waltham, MA, USA), flatmounted onto a microscope slide, and imaged using a Nikon upright epifluorescence microscope. ..

    Article Title: Interleukin-1 alpha and high mobility group box-1 secretion in polyinosinic:polycytidylic-induced colorectal cancer cells occur via RIPK1-dependent mechanism and participate in tumourigenesis
    Article Snippet: .. DAPI (excitation 358 nm, emission 461 nm) and Alexa Fluor® 488 (excitation 495 nm, emission 519 nm) fluorescent images were visualized using an upright epifluorescence microscope (Nikon TI-E, Japan). ..

    Article Title: Astrocyte Cell Surface Antigen 2 and Other Potential Cell Surface Markers of Enteric glia in the Mouse Colon
    Article Snippet: Then, the slices were rinsed 3 times with PBST for 15 min and immersed with secondary antibodies for 2 h. Lastly, the slices were washed twice in PBST and twice in PBS, mounted on slides with Fluoromount-G® (SouthernBiotech, 0100-01), and imaged. .. Epifluorescence images were acquired through the 10x [Plan, 0.25 numerical aperture (n.a.)], 20x (PlanApo, 0.75 n.a.), and 40x (PlanFluor, 0.75 n.a.) objectives of an upright epifluorescence microscope (Nikon Eclipse Ni, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging). .. Confocal images were acquired through the 40x (UPLFL, 1.3 n.a.) oil immersion objective of an inverted Olympus Fluoview FV1000 microscope (Olympus, Center Valley, PA).

    Article Title: Enteric glial adenosine 2B receptor signaling mediates persistent epithelial barrier dysfunction following acute DSS colitis
    Article Snippet: .. Epifluorescence images were acquired through the ×20 [(PlanApo, 0.75 numerical aperture (n.a.)] and ×40 (PlanFluor, 0.75 n.a.) objectives of an upright epifluorescence microscope (Nikon Eclipse Ni, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging). .. Confocal images were acquired through the ×40 (UPLFL, 1.3 n.a.) and ×60 (Plan-Apochromat, 1.42 n.a.) oil immersion objectives of an inverted Olympus Fluoview FV1000 microscope (Olympus, Center Valley, PA).

    Article Title: Enteric glial adenosine 2B receptor signaling mediates persistent epithelial barrier dysfunction following acute DSS colitis.
    Article Snippet: .. Epifluorescence images were acquired through the ×20 [(PlanApo, 0.75 numerical aperture (n.a.)] and ×40 (PlanFluor, 0.75 n.a.) objectives of an upright epifluorescence microscope (Nikon Eclipse Ni, Melville, NY) with a Retiga 2000R camera (QImaging, Surrey, BC, Canada) controlled by QCapture Pro 7.0 (QImaging). .. Confocal images were acquired through the ×40 (UPLFL, 1.3 n.a.) and ×60 (Plan-Apochromat, 1.42 n.a.) oil immersion objectives of an inverted Olympus Fluoview FV1000 microscope (Olympus, Center Valley, PA).

    Article Title: Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
    Article Snippet: .. The retinas were double-labeled with anti- RNAbinding protein with multiple splicing (RBPMS, 1:500 dilution, GeneTex, Irvine, CA, USA) and anti-beta-tubulin III (TUJ1, 1:1000 dilution, BioLegend, San Diego, CA, USA) primary antibodies, incubated in appropriate secondary antibodies (1:1000 dilution donkey antirabbit 555, and 1:1000 dilution donkey anti-mouse 488, ThermoFisher, Waltham, MA, USA), flatmounted onto a microscope slide, and imaged using a Nikon upright epifluorescence microscope. ..

    Article Title: Green‐Based Modifications of Polyethylene Surfaces Enhancing Antifouling Properties in Water‐Related Systems
    Article Snippet: .. Next, the surfaces were washed twice with PBS and imaged in an upright epifluorescence microscope (Nikon NiE) equipped with a 20× air objective, and a monochrome camera (Orca R2, Hamamatsu). ..

    Fluorescence:

    Article Title: Increased TRPV4 channel expression enhances and impairs blood vessel function in hypertension
    Article Snippet: All arteries used for experimentation had a luminal diameter ∼150 μm, and were perfused with PSS (37°C) at a rate of 1.5 ml min - using a Gilson Minipuls 3 peristaltic pump. .. Intact mesenteric artery endothelial cell Ca 2+ activity was recorded at 10 Hz using one of three imaging systems optimized for high-resolution Ca 2+ imaging: an upright epifluorescence microscope (described above; FN-1, Nikon, Japan) equipped with a 16X 0.8 NA objective lens, and two inverted fluorescence microscopes (TE300 or Ti-Eclipse, Nikon, Japan) each equipped with 40X and 100X oil-immersion (1.3 NA; S Fluor) objective. ..

    Incubation:

    Article Title: Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
    Article Snippet: .. The retinas were double-labeled with anti- RNA-binding protein with multiple splicing (RBPMS, 1:500 dilution, GeneTex, Irvine, CA, USA) and anti-beta-tubulin III (TUJ1, 1:1000 dilution, BioLegend, San Diego, CA, USA) primary antibodies, incubated in appropriate secondary antibodies (1:1000 dilution donkey anti-rabbit 555, and 1:1000 dilution donkey anti-mouse 488, ThermoFisher, Waltham, MA, USA), flatmounted onto a microscope slide, and imaged using a Nikon upright epifluorescence microscope. ..

    Article Title: Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
    Article Snippet: .. The retinas were double-labeled with anti- RNAbinding protein with multiple splicing (RBPMS, 1:500 dilution, GeneTex, Irvine, CA, USA) and anti-beta-tubulin III (TUJ1, 1:1000 dilution, BioLegend, San Diego, CA, USA) primary antibodies, incubated in appropriate secondary antibodies (1:1000 dilution donkey antirabbit 555, and 1:1000 dilution donkey anti-mouse 488, ThermoFisher, Waltham, MA, USA), flatmounted onto a microscope slide, and imaged using a Nikon upright epifluorescence microscope. ..



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